Organoids grown in the presence of microplastic, stained with phalloidin -Alexa Fluor 488 (green) to label F-actin, DAPI (red), and polyethylene terephthalate PET visualised by autofluorescence (blue). 3D reconstruction from:

1. confocal and
2. light-sheet fluorescence microscopy (LSFM) z-stacks
3. Scanning Electron Microscopy of organoids grown in the presence of polycaprolactone PCL